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Merck

539578

PKG, Iα, Bovine Lung

Synonym(s):

cGMP-Dependent Protein Kinase Catalytic Subunit, PKG Iα Catalytic Subunit, Protein Kinase G Iα, cGMP-Dependent Protein Kinase Catalytic Subunit, PKGIα Catalytic Subunit, Protein Kinase G Iα

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About This Item

NACRES:
NA.42
UNSPSC Code:
12352202

Key Documents

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form

liquid

specific activity

≥1300 units/μg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

Quality Level

100

General description

Note: 4µg = 6000 units.
Native PKGIα purified from bovine lung. PKG is involved in regulation of smooth muscle relaxation, platelet function, and cell division. PKG is activated by binding of cGMP to regulatory subunits within the holoenzyme, but in contrast to PKA, the activation of PKG does not invlove physical dissociation of catalytic from the regulatory domains. The activated enzyme phosphorylates target proteins at serine and/or threonine residues that are found within the recognition motif, Arg-Lys-Arg-Ser*-Arg-Ala-Glu.

Disclaimer

Toxicity: Standard Handling (A)

Other Notes

Brophy, M.C., et al. 2006. J. Vasc. Res.39, 95.
Colbran J.L., et al. 1992. J. Biol. Chem.267, 9589.
Corbin, J.D. and Doskeland, S.O. 1983. J Biol Chem258, 11391.
Witt, J.J. and Roskoski, R., Jr, 1975. Anal Biochem66, 253.
One unit is defined as the amount of enzyme required to incorporote 1 pmol phosphate into the synthetic heptapeptide substrate, RKRSRAE, in 1 min at 30°C.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 25 mM β-mercaptoethanol, 10 mM K₂HPO₄, 1 mM EDTA, 12% sucrose, pH 6.8.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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