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539655

Sigma-Aldrich

PKCβI, Human, Recombinant, S. frugiperda

Synonym(s):

Protein Kinase C βI, Protein Kinase CβI

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About This Item

UNSPSC Code:
12352202

Assay

≥95% (SDS-PAGE)

Quality Level

form

liquid

specific activity

≥800 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−70°C

General description

Full-length, recombinant, human PKCβI expressed in S. frugiperda insect cells. Suitable for studies of PKCβI and its regulation. PKCβI is a Ca2+ and phospholipid-dependent enzyme.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON X-100 Detergent, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Newton, A.C. 1995. J. Biol. Chem.270, 28495.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem.266, 4661.
One unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone H1 per min at 30°C, pH 7.4.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Standard Handling (A)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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