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551826

QUIN 2, Tetrapotassium Salt

Name: QUIN 2, Tetrapotassium Salt
Brand: MM

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

Synonym(s):

QUIN 2, Tetrapotassium Salt, 2-{[2- bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8- bis(carboxymethyl)aminoquinoline, 4K, 2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K

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About This Item

Empirical Formula (Hill Notation):

C₂₆H₂₃N₃O₁₀ · 4K

CAS Number:

73630-23-6

Molecular Weight:

693.87

UNSPSC Code:

12352302

NACRES:

NA.25

MDL number:

MFCD00065487

Assay:

≥95% (HPLC)

Form:

solid

Solubility:

water: 10 mg/mL

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Properties

Quality Segment

100

assay

≥95% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

yellow

solubility

water: 10 mg/mL

fluorescence

λ_ex 332 nm (high), λ_ex 354 nm (low), λ_em 505 nm (high), λ_em 510 nm (low)

shipped in

ambient

storage temp.

−20°C

SMILES string

[K+].[K+].[K+].[K+].N(CC(=O)[O-])(CC(=O)[O-])c1c(cc(cc1)C)OCc2nc3c(cc(cc3N(CC(=O)[O-])CC(=O)[O-])OC)cc2

InChI

1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4

InChI key

XAGUNWDMROKIFJ-UHFFFAOYSA-J

Description

General description

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Biochem/physiol Actions

Cell permeable: no

Primary Target
Calcium Probes

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c