Key Documents
Assay
≥90% (SDS-PAGE)
Quality Level
form
liquid
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
capacity
≥400 mmol/mol binding capacity (GTPγS)
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in immunoblotting analysis and as an affinity precipitation reagent using immobilized glutathione resin.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 20 mM HEPES, 5 mM MgCl₂, 40% glycerol, 0.05% NaN₃, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Ridley, A. 1996. Curr. Biol. 6, 1256.
Ridley, A. and Hall, A. 1992. Cell 70, 389.
Ridley, A. and Hall, A. 1992. Cell 70, 389.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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