557521
Ro-31-8220
InSolution, ≥95%, protein kinase inhibitor
Synonym(s):
InSolution Ro-31-8220, 3-[1-[3-(Amidinothio)propyl-1H-indol-3-yl]-3-(1-methyl-1H-indol-3-yl)maleimide, Bisindolylmaleimide IX, Methanesulfonate
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About This Item
Empirical Formula (Hill Notation):
C25H23N5O2S · xCH4O3S
Molecular Weight:
457.55 (free base basis)
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
liquid
Quality level:
Storage condition:
OK to freeze, protect from light
Quality Level
assay
≥95% (HPLC)
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
clear orange-brown
shipped in
wet ice
storage temp.
−20°C
General description
A competitive, selective inhibitor for protein kinase C (IC50 = 10 nM) over protein kinase A (IC50 = 900 nM), Ca2+/calmodulin-dependent protein kinase (IC50 = 17 µM) and phosphorylase protein kinase. Inhibits TPA induced p47 phosphorylation in platelets (IC50 = 700 nM). Also inhibits CD3 downregulation in T cells (IC50 = 500 nM).
Biochem/physiol Actions
Cell permeable: yes
Primary Target
PKC
PKC
Product competes with ATP.
Reversible: yes
Target IC50: 10 nM against protein kinase C (PKC); 6.8 nM against GSK-3 in primary adipocytes
Packaging
Packaged under inert gas
Physical form
A 5 mM (500 µg/181 µl) solution of Ro-31-8220 (Cat. No. 557520) in H₂O.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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