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Merck

557521

Ro-31-8220

InSolution, ≥95%, protein kinase inhibitor

Synonym(s):

InSolution Ro-31-8220, 3-[1-[3-(Amidinothio)propyl-1H-indol-3-yl]-3-(1-methyl-1H-indol-3-yl)maleimide, Bisindolylmaleimide IX, Methanesulfonate

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About This Item

Empirical Formula (Hill Notation):
C25H23N5O2S · xCH4O3S
Molecular Weight:
457.55 (free base basis)
UNSPSC Code:
12352200

Key Documents

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Product Name

Ro-31-8220, InSolution, ≥95%, protein kinase inhibitor

assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

clear orange-brown

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Cell permeable: yes
Primary Target
PKC
Product competes with ATP.
Reversible: yes
Target IC50: 10 nM against protein kinase C (PKC); 6.8 nM against GSK-3 in primary adipocytes

Disclaimer

Toxicity: Standard Handling (A)

General description

A competitive, selective inhibitor for protein kinase C (IC50 = 10 nM) over protein kinase A (IC50 = 900 nM), Ca2+/calmodulin-dependent protein kinase (IC50 = 17 µM) and phosphorylase protein kinase. Inhibits TPA induced p47 phosphorylation in platelets (IC50 = 700 nM). Also inhibits CD3 downregulation in T cells (IC50 = 500 nM).

Packaging

Packaged under inert gas

Physical form

A 5 mM (500 µg/181 µl) solution of Ro-31-8220 (Cat. No. 557520) in H₂O.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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