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559240

Sigma-Aldrich

Anti-rSec6 Mouse mAb (9H5)

liquid, clone 9H5, Calbiochem®

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

9H5, monoclonal

form

liquid

does not contain

preservative

species reactivity

chicken, rabbit, bovine, human, monkey, mouse, rat, canine, porcine, hamster

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Exoc5(60627)

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa rSec6 protein.
Recognizes the ~87 kDa rSec6 protein in PC12 cells.
This Anti-rSec6 Mouse mAb (9H5) is validated for use in Immunoblotting, Immunofluorescence for the detection of rSec6.

Immunogen

Rat
recombinant, rat rSec6 fused to a His•Tag sequence

Application

Immunoblotting (4 µg/ml, chemiluminescence)

Immunofluorescence (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
ESK-4 cells

Other Notes

Immunofluorescence of PC12 cells using this antibody shows colocalization of rSec6 with vesicles and sites of exocytosis. Variables associated with assay conditions will dictate the proper working dilution.
Kee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14438.
Hsu, S.-C., et al. 1996. Neuron17, 1209.
TerBush, D.R., et al. 1995. J. Cell Biol.130, 299.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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