559240
Anti-rSec6 Mouse mAb (9H5)
liquid, clone 9H5, Calbiochem®
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About This Item
UNSPSC Code:
12352203
biological source
mouse
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
9H5, monoclonal
form
liquid
does not contain
preservative
species reactivity
chicken, rabbit, bovine, human, monkey, mouse, rat, canine, porcine, hamster
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... Exoc5(60627)
General description
Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~87 kDa rSec6 protein.
Recognizes the ~87 kDa rSec6 protein in PC12 cells.
This Anti-rSec6 Mouse mAb (9H5) is validated for use in Immunoblotting, Immunofluorescence for the detection of rSec6.
Immunogen
Rat
recombinant, rat rSec6 fused to a His•Tag sequence
Application
Immunoblotting (4 µg/ml, chemiluminescence)
Immunofluorescence (see comments)
Immunofluorescence (see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
ESK-4 cells
ESK-4 cells
Other Notes
Immunofluorescence of PC12 cells using this antibody shows colocalization of rSec6 with vesicles and sites of exocytosis. Variables associated with assay conditions will dictate the proper working dilution.
Kee, Y., et al. 1997. Proc. Natl. Acad. Sci. USA94, 14438.
Hsu, S.-C., et al. 1996. Neuron17, 1209.
TerBush, D.R., et al. 1995. J. Cell Biol.130, 299.
Hsu, S.-C., et al. 1996. Neuron17, 1209.
TerBush, D.R., et al. 1995. J. Cell Biol.130, 299.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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