Skip to Content
Merck

596200

Thioflavin T

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils.

Synonym(s):

Thioflavin T, ThT, Thioflavine T, Basic Yellow 1

Sign In to View Organizational & Contract Pricing.

Select a Size

All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C17H19ClN2S
CAS Number:
2390-54-7
Molecular Weight:
318.86
UNSPSC Code:
12171500
NACRES:
NA.25
MDL number:
MFCD00011944

Product Name

Thioflavin T, A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils.

color

yellow

SMILES string

S1c2c(ccc(c2)C)N(C1=C3C=CC(=[N+](C)C)C=C3)C.[Cl-]

InChI

1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

JADVWWSKYZXRGX-UHFFFAOYSA-M

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

solubility

water: 5 mg/mL

fluorescence

λex ~385 nm (free)
λex ~437 nm
λem ~445 nm (free)
λem ~485 nm

shipped in

ambient

storage temp.

15-25°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Exhibits enhanced fluorescence upon binding to amyloid fibrils
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Standard Handling (A)

General description

A cell-permeable benzothiazole dye that exhibits enhanced fluorescence upon binding to amyloid fibrils. Useful in monitoring stacked β sheet aggregates. Associates with aggregated fibrils of synthetic β/A4-derived peptides β(1-28) and β(1-40), resulting in a shift in the excitation maximum and an enhanced emission. Excitation max.: ~385 nm (free); ~437 nm (bound); emission max.: ~445 nm (free); ~485 nm (bound).

Other Notes

Tjernberg, L.O., et al. 1999. J. Biol. Chem.274, 12619.
Naiki, H. and Nakakuki, K. 1996. Lab. Invest.74, 374.
LeVine, H. 1993. Protein Sci.2, 404.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service