TACE Substrate III, Fluorogenic

Name: TACE Substrate III, Fluorogenic
Brand: MM

A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (Km = 19 µM).

Synonym(s):

TACE Substrate III, Fluorogenic, TNF-α Convertase Enzyme Substrate II, Fluorogenic, ADAM-17 Substrate II, Fluorogenic, DABCYL-LAQAVRSSSR-EDANS, TNF-α Convertase Enzyme Substrate II, Fluorogenic, DABCYL-LAQAVRSSSR-EDANS, ADAM-17 Substrate II, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):

C₇₀H₁₀₄N₂₂O₁₈S

CAS Number:

396716-14-6

Molecular Weight:

1573.78

UNSPSC Code:

12352200

Product Name

TACE Substrate III, Fluorogenic, A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (Km = 19 µM).

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
protect from light

color

red

solubility

50% acetic acid: 5 mg/mL
DMSO: 5 mg/mL

fluorescence

λ_ex ~340 nm
λ_em ~490 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Biochem/physiol Actions

Primary Target
A fluorescence resonance energy transfer (FRET) peptide substrate for TACE

Product does not compete with ATP.

Reversible: no

Cell permeable: no

Km = 19 µM as a fluorescence resonance energy transfer (FRET) peptide substrate for TACE

Disclaimer

Toxicity: Standard Handling (A)

General description

A fluorescence energy transfer (FRET) peptide substrate for tumor necrosis factor (TNF)-α convertase (TACE) (K_m ~19 µM). Cleavage occurs between the alanine and valine residues. Useful for screening TACE inhibitors. Excitation max: ~340 nm; emission max: ~490 nm.

A fluorescence resonance energy transfer (FRET) peptide substrate for TACE (K_m = 19 µM). Substrate cleavage occurs at the Ala-Val bond. Useful for the screening of TACE and SARS protease 3-CL^PRO inhibitors.

Other Notes

Bacha, U., et al. 2004. Biochemistry43, 4906.
Patel, I.R., et al. 1998. J. Immunol.160, 4570.

DABCYL-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-EDANS

Packaging

Yes

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c