616407
TACE Substrate IV, Fluorogenic
Synonym(s):
TACE Substrate IV, Fluorogenic, TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
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About This Item
Empirical Formula (Hill Notation):
C59H93N23O20
Molecular Weight:
1444.51
NACRES:
NA.77
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Quality level:
Storage condition:
OK to freeze, desiccated, protect from light
assay
≥95% (HPLC)
Quality Level
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated, protect from light
color
yellow
solubility
DMSO: 5 mg/mL
fluorescence
λex ~320 nm, λem ~420 nm
shipped in
ambient
storage temp.
2-8°C
General description
An internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (kcat = 21.6 s-1 when the substrate concentration is half the Vmax, S0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.
An internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity
Fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity
Product does not compete with ATP.
Reversible: no
kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Abz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
Jin, G., et al. 2002. Anal. Biochem.302, 269.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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