648480
Troponin I, Human Heart
Synonym(s):
Troponin I, Human Heart, hcTnI
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About This Item
assay
≥95% (SDS-PAGE)
Quality Segment
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
urea/Tris buffer (0.02 M Tris, HCl, pH 7.5, 7 M urea, 5 mM EDTA, 15 mM 2-mercaptoethanol): 1 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
Native troponin I purified from human heart. Inhibitory subunit of troponin that binds to actin in the absence of Ca2+, thus inhibiting the ATPase activity of actomyosin. Presence of hcTnI in plasma has been used for early detection of myocardial infarction. Useful as a standard in immunoassay and as an immunogen for antisera production. Also useful as a tracer for iodination.
Physical form
Lyophilized from 10 mM HCl.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Other Notes
Falahati, A., et al. 1999. Am. Heart J. 137, 332.
Mangano, D.T. 1994. Anesthesiology 81, 1317.
Mangano, D.T. 1994. Anesthesiology 81, 1317.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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