662065
Ubiquitin, Methylated, Human, Recombinant
Synonym(s):
Me-Ub
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About This Item
NACRES:
NA.77
UNSPSC Code:
12352202
Form:
lyophilized solid
Assay:
≥95% (SDS-PAGE)
assay
≥95% (SDS-PAGE)
Quality Level
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
solubility
aqueous buffer: 50 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant, human methylated ubiquitin. A derivative of ubiquitin in which lysine residues are reductively methylated. Methylated ubiquitin can ligate to protein substrates, but does not form polyubiquitin chains. Protein breakdown rates obtained with methylated ubiquitin are slower than those obtained with unmethylated ubiquitin. Useful for reducing the polyubiquitin chain length and for determining rates of ubiquitin conjugation.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Hershko, A. and Heller, H. 1991. J. Biol. Chem.266, 16376.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.
Hershko, A. and Heller, H. 1985. Biochem. Biophys. Res. Commun.128, 1079.
Jentoft, N. and Dearborn, D.G. 1983. Methods. Enz.91, 570.
Jentoft, N. and Dearborn, D.G. 1980. Anal. Biochem.106, 186.
Jentoft, N. and Dearborn, D.G. 1979. J. Biol. Chem.254, 4359.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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