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Merck

676472

VEGF165, Human, Recombinant, S. frugiperda

Synonym(s):

Vascular Endothelial Growth Factor165, rhVEGF, rhVPF, rh-Vasculotropin

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About This Item

UNSPSC Code:
12352202

assay

≥97% (SDS-PAGE)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤1 EU/μg Endotoxin (EU/μg VEGF)

shipped in

ambient

storage temp.

−70°C

Quality Level

Biochem/physiol Actions

ED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells

Disclaimer

Toxicity: Standard Handling (A)

General description

Recombinant, human VEGF165 (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.

Other Notes

Liu, X., et al. 2001. J. Urology166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res.5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.

Physical form

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.

Preparation Note

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
Reconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3


Certificates of Analysis (COA)

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