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Merck

676602

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

lyophilized, clone 16C2, Calbiochem®

Synonym(s):

Anti-Vasodilator-stimulated Phosphoprotein, Activated

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About This Item

UNSPSC Code:
12352203

Product Name

PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2), lyophilized, clone 16C2, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

16C2, monoclonal

form

lyophilized

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer239/pSer239)

Quality Level

100

Analysis Note

Positive Control
Vanadate-treated A431 cells or 8-pCPT-treated platelets

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)

Immunocytochemistry (1-10 µg/ml)

Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

Disclaimer

Toxicity: Standard Handling (A)

General description

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
This PhosphoDetect Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

Immunogen

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH

Other Notes

Does not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton™ X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton X-100 detergent, and 0.05% Tween-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost.82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem.273, 20029.

Physical form

Lyophilized from 2X PBS, PEG, sucrose.

Preparation Note

Reconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Loek van Heerebeek et al.
Circulation, 126(7), 830-839 (2012-07-19)
Prominent features of myocardial remodeling in heart failure with preserved ejection fraction (HFPEF) are high cardiomyocyte resting tension (F(passive)) and cardiomyocyte hypertrophy. In experimental models, both reacted favorably to raised protein kinase G (PKG) activity. The present study assessed myocardial

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