70527
100 mM IPTG Solution - Novagen
Convenient, ready-to-use solution
Synonym(s):
100 mM IPTG Solution - Novagen, IPTG Solution, 100 mM, Isopropyl -β-D-1-thiogalactopyranoside, Isopropylthio -β-D-galactoside
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About This Item
Form:
solution
Quality level:
Storage condition:
OK to freeze, avoid repeated freeze/thaw cycles
Quality Level
form
solution
mol wt
238.30 g/mol
manufacturer/tradename
Novagen®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
shipped in
wet ice
storage temp.
−20°C
SMILES string
S([C@@H]1O[C@@H]([C@@H]([C@@H]([C@H]1O)O)O)CO)C(C)C
InChI
1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChI key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
Application
100 mM IPTG Solution - Novagen has been used to:
- induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
- induce expression in calcium-independent sortase from BL21(DE3) cells
- turn on bacterial protein production of the protein A/G-MNase
Biochem/physiol Actions
Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Carcinogenic / Teratogenic (D)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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