PhosphoSafe Extraction Reagent

Name: PhosphoSafe Extraction Reagent
Brand: MM

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Synonym(s):

Protein Extraction Reagent

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About This Item

NACRES:

NA.77

UNSPSC Code:

41106510

Product Name

PhosphoSafe Extraction Reagent, Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

form

liquid

manufacturer/tradename

Novagen^®

storage condition

OK to freeze

technique(s)

immunoblotting: suitable

application(s)

sample preparation

shipped in

dry ice

storage temp.

−70°C

Quality Level

100

Related Categories

Cell Lysis Buffers & Protein Extraction Reagents

Protein Sample Prep

Disclaimer

Toxicity: Toxic (F)

Features and Benefits

Phosphorylation state preserved during extraction
Compatible with kinase assays

General description

PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Other Notes

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dual Oxidase-Induced Sustained Generation of Hydrogen Peroxide Contributes to Pharmacologic Ascorbate-Induced Cytotoxicity.

Adrienne R Gibson et al.

Cancer research, 80(7), 1401-1413 (2020-02-12)

Pharmacologic ascorbate treatment (P-AscH-, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is