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Merck

71772

Millipore

ProteoExtract® Transmembrane Protein Extraction Kit

Synonym(s):

Membrane Protein Isolation Kit

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.77

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

2-8°C

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General description

The ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).

Application

ProteoExtract® Transmembrane Protein Extraction Kit has been used: for extraction of protein from the apex segment of the colon in western blot analysis of Na+/H+ exchanger (NHE3) for extracting plasma membrane proteins

Features and Benefits

  • No sonication, intensive vortexing, lengthy ultracentrifugation, or high-temperature incubation is needed.
  • Reagents are sufficient to process a total of 40 samples.

Other Notes

Nakamura, E., et al. 2008. Cancer Sci.99, 1390.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Toxic (F)

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Impairment of electroneutral Na+ transport and associated downregulation of NHE3 contributes to the development of diarrhea following in vivo challenge with Brachyspira spp.
Cole B. Enns, et al.
The American Journal of Physiology, 318(2), G288?G297-G288?G297 (2020)
Hepatic Tmem30a Deficiency Causes Intrahepatic Cholestasis by Impairing Expression and Localization of Bile Salt Transporters
Liu L, et al.
The American Journal of Pathology, 187(12), 2775-2787 (2017)
Runtao Ding et al.
Frontiers in behavioral neuroscience, 14, 60-60 (2020-05-05)
The monocarboxylate transporters (MCTs) MCT1, MCT2, and MCT4 are essential components of the astrocyte-neuron lactate shuttle (ANLS), which is a fundamental element of brain energetics. Decreased expression of MCTs can induce cognitive dysfunction of the brain. In the present study
Huimin Luo et al.
Oncology reports, 42(3), 1237-1247 (2019-07-20)
Although cisplatin is one of the most accepted therapies for ovarian cancer, recurrence and drug resistance remain problematic. Both the ubiquitin‑proteasome system (UPS) and connexin (Cx) are closely related to tumor progression. However, the role of ubiquitin‑specific protease 14 (USP14) and Cx
Asahi Takeuchi et al.
Nutrients, 12(9) (2020-09-24)
IIAEK (Ile-Ile-Ala-Glu-Lys, lactostatin) is a novel cholesterol-lowering pentapeptide derived from bovine milk β-lactoglobulin. However, the molecular mechanisms underlying the IIAEK-mediated suppression of intestinal cholesterol absorption are unknown. Therefore, we evaluated the effects of IIAEK on intestinal cholesterol metabolism in a
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