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Merck

72158

BacMagic-2 Transfection Kit - Novagen

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About This Item

NACRES:
NA.54
UNSPSC Code:
41106617

manufacturer/tradename

Novagen®

storage condition

OK to freeze

Quality Level

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS

General description

The BacMagic-2 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Like BacMagic DNA, BacMagic-2 DNA is derived from an AcNPV genome, contains an ORF 1629 deletion which prevents nonrecombinant virus from replicating in insect cells, and contains a deletion of the nonessential chitinase (chiA) gene, to improve production of secreted and membrane-targeted proteins. BacMagic-2 DNA have an additional deletion of the v-cath gene, a cathespsin-like cysteine protease, resulting in significantly improved quality and yield for most target proteins. The BacMagic-2 Transfection Kit contains the BacMagic-2 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. In addition, it contains Sf9 cells and BacVector Medium, for optimal transfection performance.
Not available for sale in Japan

Other Notes

  • 0.5 µg : BacMagic-2 DNA
  • 50 µl : Insect GeneJuice Transfection Reagent
  • 2 x 1: mlSf9 Cells
  • 1 L: BacVector Insect Cell Medium
  • 2 µg : Transfection Control Plasmid

Preparation Note

Ship Code: Multiple Storage ConditionsStorage: Multiple storage requirements

Legal Information

GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
72158-304055977266481

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