ABE1965
Anti-CEP192
from rabbit
Synonym(s):
Centrosomal protein of 192 kDa
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
FACS, IF
Citations:
-
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
flow cytometry: suitable, immunofluorescence: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... CEP192(55125)
General description
279.11 kDa calculated.
Centrosomal protein of 192 kDa (UniProt: Q8TEP8; also known as Cep192) is encoded by the CEP192 (also known as KIAA1569, PP8407) gene (Gene ID: 55125) in human. Cep192 is localized to the pericentriolar region in mitotic centrosomes. It is required for mitotic centrosome and spindle assembly and appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication. It stimulates the formation of the scaffolding upon which gamma tubulinring complexes and other proteins involved in microtubule nucleation and spindle assembly become functional during mitosis. Cep192 is reported to bind Aurora A and Plk1 and targets them to centrosomes in a pericentrin-dependent manner, and promotes sequential activation of both kinases via T-loop phosphorylation. The Cep192-bound Plk1 then phosphorylates Cep192 at several residues, thereby generating attachment sites for the gamma-tubulin ring complex and other pericentriolar material components, which promotes their recruitment and subsequent microtubule nucleation. (Ref.: Joukov, V et al. (2014). Mol. Cell 55(4): 578 591).
Immunogen
His-tagged recombinant fragment corresponding to 299 amino acids from the C-terminal region of human Centrosomal protein of 192 kDa (CEP192).
Application
Anti-CEP192, Cat. No. ABE1965, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 192 kDa and has been tested in Flow Cytometry and Immunofluorescence.
Immunofluorescence Analysis: A representative lot detected CEP192 in U2OS cells (Park, S.Y., et. al. (2014). Nat Struct Mol Biol. 21(8):696-703).
Biochem/physiol Actions
This polyclonal antibody detects CEP192 in human. It targets an epitope within 299 amino acids from the C-terminal region.
Physical form
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 20 mM Tris-Cl (pH 8.0), 50% ethylene glycol without azide.
Analysis Note
Evaluated by Flow Cytometry in U2OS cells.
Flow Cytometry Analysis: 0.1 µg of this antibody detected CEP192 in one million U2OS cells.
Flow Cytometry Analysis: 0.1 µg of this antibody detected CEP192 in one million U2OS cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
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signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - STOT RE 2 Oral
target_organs
Kidney
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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