Anti-Daple Antibody, CT

Concentration: Please refer to lot specific datasheet.

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in lysate from Daple-transfected COS7 cells.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected the expression of Myc-tagged full-length human Daple in lysate from transfected COS-7 cells.

Detect Daple using this rabbit polyclonal Anti-Daple, CT, Cat. No. ABS515, validated for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:300 dilution from a representative lot detected exogenously expressed human Daple in paraformaldehyed-fixed HEK293 transfectants (Courtesy of Pradipta Ghosh, M.D, UC San Diego, U.S.A.).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected a ~260 kDa target band in lysate from control shRNA-, but not Daple shRNA-, treated HeLa cells (Courtesy of Pradipta Ghosh, M.D, UC San Diego, U.S.A.).

Western Blotting Analysis: A representative lot detected exogenously expressed human Daple in lysates from various transfected cells (Aznar, N., et al. (2015). Elife. 4:e07091).

Research Category
Signaling

This rabbit polyclonal antibody targets the region between the coiled-coils and DVL1-/PDZ-binding domain. Target region is present in all three human Daple spliced isoforms reported by UniProt (Q9P219). Specificiy of target band detection was verified using lysates from Daple shRNA-treated cells.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Daple (UniProt Q9P219; also known as Hook-related protein 2, HkRP2, hDaple, Coiled-coil domain-containing protein 88C, Dvl-associating protein with a high frequency of leucine residues) is encoded by DAPLE (also known as KIAA1509, SCA40) gene (Gene ID 440193) in human. It acts as a negative regulator of the canonical Wnt signaling pathway and acts downstream of DVL to inhibit CTNNB1/beta-catenin stabilization. Daple has a PDZ-domain binding motif in its C-terminus with which it interacts with the DVL protein. Daple also contains a Gα-binding and activating motif, which activates Gαi proteins and an adjacent domain that directly binds frizzled receptors, thereby linking Wnt stimulation to G protein activation. This triggers non-canonical Wnt responses and may suppress the beta-catenin/TCF/LEF pathway and tumorigenesis, but can enhance PI3K-Akt and Rac1 signals by releasing beta/gamma subunits and may contribute to tumor cell invasiveness. Mutations in this DAPLE gene are known to cause autosomal recessive, primary non-syndromic congenital hydrocephalus.

~260 kDa observed. 228.2/57.53/49.25 kDa (isoform 1/2/3) calculated. Uncharacterized bands may be observed in some lysate(s).

His-tagged recombinant human Daple fragment from the region between the coiled-coils and DVL1-/PDZ-binding domain.

Stable for 1 year at 2-8°C from date of receipt.