ABS787

Anti-PGK1 Antibody (Center)

Name: Anti-PGK1 Antibody (Center)
Brand: MM

from rabbit

Synonym(s):

PGK1, PGKA, Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rabbit

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

P00558

shipped in

dry ice

Gene Information

human ... PGK1(5230)

General description

Phosphoglycerate kinase 1 (PGK1) and encoded by the gene names Pgk1 or Pgk-1, is important for phosphoprotein glycolysis. PGK1 is an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway that is often highly expressed in prostate cancer and is regulated by hypoxia-inducible factor-1α. Extracellular PGK1 facilitates the cleavage of plasminogen generating the vascular inhibitor angiostatin.

~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: Central region

KLH-conjugated synthetic peptide corresponding to the central region of human PGK1.

Application

Detect PGK1 using this rabbit polyclonal Anti-PGK1 Antibody, Cat. No. ABS787, validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, and Flow Cytometry.

Research Category
Metabolism

Research Sub Category
Glucose/Glycogen Metabolism

Western Blotting Analysis (WB): A 1:1000 dilution from a representative lot of this antibody detected PGK1 in multiple lysates. See chart for reactivity detail.

Immunohistochemistry Analysis (IHC): A 1:10-50 dilution from a representative lot detected PGK1 in human cancer tissue.

Immunocytochemistry Analysis (ICC): A 1:10-50 dilution from a representative lot detected PGK1 in HeLa cells.

Flow Cytometry (FC): A 1:10-50 dilution of this antibody detected PGK1 in HeLa cells.

Physical form

Ammonium Sulfate

Format: Purified

Purified rabbit polyclonal in buffer containing PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Evaluated by Western Blotting in HT1376 cell lysate.

Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected PGK1 in HT1376 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Bioenergetic status modulates motor neuron vulnerability and pathogenesis in a zebrafish model of spinal muscular atrophy.

Penelope J Boyd et al.

PLoS genetics, 13(4), e1006744-e1006744 (2017-04-21)

Degeneration and loss of lower motor neurons is the major pathological hallmark of spinal muscular atrophy (SMA), resulting from low levels of ubiquitously-expressed survival motor neuron (SMN) protein. One remarkable, yet unresolved, feature of SMA is that not all motor