Anti-PGK1 Antibody (Center)

Phosphoglycerate kinase 1 (PGK1) and encoded by the gene names Pgk1 or Pgk-1, is important for phosphoprotein glycolysis. PGK1 is an ATP-generating glycolytic enzyme that forms part of the glycolytic pathway that is often highly expressed in prostate cancer and is regulated by hypoxia-inducible factor-1α. Extracellular PGK1 facilitates the cleavage of plasminogen generating the vascular inhibitor angiostatin.

~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Epitope: Central region

KLH-conjugated synthetic peptide corresponding to the central region of human PGK1.

Detect PGK1 using this rabbit polyclonal Anti-PGK1 Antibody, Cat. No. ABS787, validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, and Flow Cytometry.

Research Category
Metabolism

Research Sub Category
Glucose/Glycogen Metabolism

Western Blotting Analysis (WB): A 1:1000 dilution from a representative lot of this antibody detected PGK1 in multiple lysates. See chart for reactivity detail.

Immunohistochemistry Analysis (IHC): A 1:10-50 dilution from a representative lot detected PGK1 in human cancer tissue.

Immunocytochemistry Analysis (ICC): A 1:10-50 dilution from a representative lot detected PGK1 in HeLa cells.

Flow Cytometry (FC): A 1:10-50 dilution of this antibody detected PGK1 in HeLa cells.

Ammonium Sulfate

Format: Purified

Purified rabbit polyclonal in buffer containing PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Evaluated by Western Blotting in HT1376 cell lysate.

Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected PGK1 in HT1376 cell lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.