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Merck

MAB1287

Sigma-Aldrich

Anti-Nuclear Ribonucleoprotein Antibody, clone 58-15

ascites fluid, clone 58-15, Chemicon®

Synonym(s):

RNP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

antibody form

ascites fluid

antibody product type

primary antibodies

clone

58-15, monoclonal

species reactivity

rat, human

should not react with

mouse

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

input

sample type neural stem cell(s)

isotype

IgM

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HNRNPH3(3189)

General description

28 kDa
snRNPs or small nuclear ribonucleoproteins, are particles that combine with pre-mRNA and various proteins to form spliceosomes (a type of large molecular complex). SnRNPs "recognize" the places along a strand of pre-mRNA and are essential in the removal of introns. These molecules are found within the cell′s nucleus. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA. These molecules are usually about 150 nucleotides long. The snRNA is bound by a Ribonuclear protein (RNP) to activate its enzymatic activity. The precise beginnings and ends of introns on the primary transcripts are marked by signals so that they can be recognized and removed. The intron-boundary signals are recognized by a short bit of RNA found in curious RNA-protein complexes known as the small nuclear ribonucleoprotein particles - snRNPs, for short. At least four different kinds of snRNPs cooperate in most splicing. The RNA in these particles is like ribosomal RNA in that it is used directly, and has both an enzymatic and a structural role.

Application

Indirect immunofluorescence: 1:20-1:30. Use 30-100 μl per well/slide.

Immunohistochemistry in formalin fixed paraffin embedded tissues: requires citrate acid microwave or HIER pretreatment: 1:20-1:30 primary antibody dilution. Archival tissues will require more antibody than 24-48 fixed specimens.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Neural Stem Cells

RNA Binding Protein (RBP)
This Anti-Nuclear Ribonucleoprotein Antibody, clone 58-15 is validated for use in IF, IH, IH(P) for the detection of Nuclear Ribonucleoprotein.

Biochem/physiol Actions

Stains nuclear ribonucleoprotein particles (RNP) in human cells. Does not cross react with mouse RNP, other species not tested. RNP are 20-80nm dense nuclear particles. Highest labeling densities are found in RNP, but heterochromatin, euchromatin and nucleoli are also stained.

Analysis Note

Control
Jurkat whole cell lysate, K-562 whole cell lysate

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pilar López-Iglesias et al.
World journal of stem cells, 3(6), 53-62 (2011-08-24)
To study the ability of human adipose-derived mesenchymal stem cells (AMSCs) to survive over the short and long term, their biodistribution and their biosafety in vivo in tumor-prone environments. We subcutaneously injected human AMSCs from different human donors into immunodeficient

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