MAB1287
Anti-Nuclear Ribonucleoprotein Antibody, clone 58-15
ascites fluid, clone 58-15, Chemicon®
Synonym(s):
RNP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
58-15, monoclonal
species reactivity
rat, human
should not react with
mouse
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
input
sample type neural stem cell(s)
isotype
IgM
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... HNRNPH3(3189)
General description
28 kDa
snRNPs or small nuclear ribonucleoproteins, are particles that combine with pre-mRNA and various proteins to form spliceosomes (a type of large molecular complex). SnRNPs "recognize" the places along a strand of pre-mRNA and are essential in the removal of introns. These molecules are found within the cell′s nucleus. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA. These molecules are usually about 150 nucleotides long. The snRNA is bound by a Ribonuclear protein (RNP) to activate its enzymatic activity. The precise beginnings and ends of introns on the primary transcripts are marked by signals so that they can be recognized and removed. The intron-boundary signals are recognized by a short bit of RNA found in curious RNA-protein complexes known as the small nuclear ribonucleoprotein particles - snRNPs, for short. At least four different kinds of snRNPs cooperate in most splicing. The RNA in these particles is like ribosomal RNA in that it is used directly, and has both an enzymatic and a structural role.
Application
Indirect immunofluorescence: 1:20-1:30. Use 30-100 μl per well/slide.
Immunohistochemistry in formalin fixed paraffin embedded tissues: requires citrate acid microwave or HIER pretreatment: 1:20-1:30 primary antibody dilution. Archival tissues will require more antibody than 24-48 fixed specimens.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry in formalin fixed paraffin embedded tissues: requires citrate acid microwave or HIER pretreatment: 1:20-1:30 primary antibody dilution. Archival tissues will require more antibody than 24-48 fixed specimens.
Optimal working dilutions must be determined by the end user.
Research Sub Category
Neural Stem Cells
RNA Binding Protein (RBP)
Neural Stem Cells
RNA Binding Protein (RBP)
This Anti-Nuclear Ribonucleoprotein Antibody, clone 58-15 is validated for use in IF, IH, IH(P) for the detection of Nuclear Ribonucleoprotein.
Biochem/physiol Actions
Stains nuclear ribonucleoprotein particles (RNP) in human cells. Does not cross react with mouse RNP, other species not tested. RNP are 20-80nm dense nuclear particles. Highest labeling densities are found in RNP, but heterochromatin, euchromatin and nucleoli are also stained.
Analysis Note
Control
Jurkat whole cell lysate, K-562 whole cell lysate
Jurkat whole cell lysate, K-562 whole cell lysate
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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