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MAB348A4

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Alexa488 Conj.

Name: Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Alexa488 Conj.
Brand: MM

clone 22C11, from mouse, ALEXA FLUOR^™ 488

Synonym(s):

Amyloid beta A4 protein, ABPP, APPI, APP, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II, N-APP, Soluble APP-alpha, S-APP-alpha, Soluble APP-beta, S-APP-beta, C99, Beta-amyloid protein 42, Beta

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

ALEXA FLUOR^™ 488

Clone:

22C11, monoclonal

Application:

ICC, IHC

Citations:

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Properties

biological source

mouse

Quality Segment

100

conjugate

ALEXA FLUOR^™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

22C11, monoclonal

species reactivity

rat, pig

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), porcine (based on 100% sequence homology), human (based on 100% sequence homology), canine (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

NP_000475

UniProt accession no.

P05067

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

Description

General description

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged human and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

Reacts with pre-A4. The unconjugated antibody (MAB348) recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000).

Immunogen

Epitope: a.a. 66-81 of APP (N-terminus)

Application

Anti-Alzheimer Precursor Protein A4 Antibody, clone 22C11, Alexa488 Conj. is an antibody against Alzheimer Precursor Protein A4 for use in IHC, ICC.

Immunohistochmeistry Analysis: A 1:50 dilution from a representative lot detected Alzheimer Precursor Protein A4 in adult mouse brain tissue.

Biochem/physiol Actions

Unconjugated MAB348 recognizes N-terminal amino acids 66-81 on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP: immature, ~110kDa; sAPP, ~120kDa; and mature, ~130kDa (Hoffmann et al., 2000). This antibody is known to cross react with APLP2 (Slunt, 1994).

Analysis Note

Evaluated by Immunocytochemistry in mouse N1E-115 neuroblastoma cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Alzheimer Precursor Protein A4 in mouse N1E-115 neuroblastoma cells.

Control
Mouse N1E-115 neuroblastoma cells

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c