MAB4308
Anti-VIN-IS-56 Antibody, clone VIN-IS-56
clone VIN-IS-56, Chemicon®, from mouse
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About This Item
eCl@ss:
32160702
UNSPSC Code:
12352203
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
VIN-IS-56, monoclonal
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
input
sample type neural stem cell(s)
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
General description
Almost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.
Immunogen
Human Embryonal carcinoma cells
Application
Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
Immunohistochemistry & Immunocytochemistry:
Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).
Recommended antibody dilution: 10-20 μg/ml
Dilution Buffer: PBS + 4% FCS
Expected staining pattern: cell surface staining
FACS Analysis:
Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells
Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde
Recommended controls: Undifferentiated human ES or EC cells stain weakly.
Immunoblotting:
Not tested
EIA:
Not tested
Optimal working dilutions must be determined by end user.
Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).
Recommended antibody dilution: 10-20 μg/ml
Dilution Buffer: PBS + 4% FCS
Expected staining pattern: cell surface staining
FACS Analysis:
Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells
Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde
Recommended controls: Undifferentiated human ES or EC cells stain weakly.
Immunoblotting:
Not tested
EIA:
Not tested
Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Neural Stem Cells
Neural Stem Cells
Biochem/physiol Actions
This monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
Analysis Note
Control
Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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