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Merck

MAB4308

Anti-VIN-IS-56 Antibody, clone VIN-IS-56

clone VIN-IS-56, Chemicon®, from mouse

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About This Item

eCl@ss:
32160702
UNSPSC Code:
12352203

Product Name

Anti-VIN-IS-56 Antibody, clone VIN-IS-56, clone VIN-IS-56, Chemicon®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

VIN-IS-56, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

input

sample type neural stem cell(s)

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

Analysis Note

Control
Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly

Application

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
Immunohistochemistry & Immunocytochemistry:

Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).

Recommended antibody dilution: 10-20 μg/ml

Dilution Buffer: PBS + 4% FCS

Expected staining pattern: cell surface staining



FACS Analysis:

Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells

Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde

Recommended controls: Undifferentiated human ES or EC cells stain weakly.



Immunoblotting:

Not tested



EIA:

Not tested



Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells

Biochem/physiol Actions

This monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Almost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.

Immunogen

Human Embryonal carcinoma cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Zhicheng Shao et al.
Nature communications, 7, 10869-10869 (2016-03-08)
It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical

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