Skip to Content
Merck

Key Documents

MABE951

Sigma-Aldrich

Anti-Histone H3.3 (H3-3A,H3-3B) Antibody

rat monoclonal, 6C4A3

Synonym(s):

Histone H3.3, H3.3A, H3F3

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Product Name

Anti-Histone H3.3 Antibody, clone 6C4A3, clone 6C4A3, 1 mg/mL, from rat

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6C4A3, monoclonal

species reactivity

human, monkey, mouse, canine

concentration

1 mg/mL

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_002098

UniProt accession no.

P84243

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... H3F3B(3021)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
~17 kDa observed

Application

Anti-Histone H3.3 Antibody, clone 6C4A3 is an antibody against Histone H3.3 for use in western blotting.
Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Histone H3.3 with recombinant H3.3 proteins. Additionally, 1 µg/mL from a representative lot detected Histone H3.3 in HeLa, COS1, NIH/3T3, NRK, and MDCK whole cell lysates (Prof. Taro Tachibana, Cell Engineering Corporation.).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Zhexin Zhu et al.
Cell stem cell, 20(2), 274-289 (2016-12-13)
The chromatin landscape and cellular metabolism both contribute to cell fate determination, but their interplay remains poorly understood. Using genome-wide siRNA screening, we have identified prohibitin (PHB) as an essential factor in self-renewal of human embryonic stem cells (hESCs). Mechanistically, PHB

Global Trade Item Number

SKUGTIN
MABE95104053252970603

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service