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Merck

MABN492

Anti-GABA(B)R1 Protein Antibody, clone N93A/49

clone N93A/49, from mouse

Synonym(s):

Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

N93A/49, monoclonal

species reactivity

mouse, rat

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_112290

UniProt accession no.

Q9Z0U4

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Gabbr1(54393)
rat ... Gabbr1(81657)

General description

Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.
~115 kDa observed. Five isoforms at 112 kDa (Isoform 1E), 108 kDa (Isoform 1A), 95 kDa (Isoform 1B), 98 kDa (Isoform 1C) and 91 kDa (Isoform 1D) are known to exist. Uncharacterized bands may be observed above ~150 kDa in some tissue lysates.

Immunogen

Recombinant protein corresponding to rat GABA(B)R1.

Application

Immunohistochemistry Analysis: A representative lot detected GABA(B)R1 in adult rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis).

Western Blotting Analysis: A representative lot detected GABA(B)R1 in adult rat brain tissue and in WT GABA(B)R1 mice, but demonstrated a loss of signal in GABA(B)R1 knockout mice.
This GABA(B)R1 Protein antibody is validated for use in WB & IHC for the detection of the GABA(B)R1 Protein protein.

Biochem/physiol Actions

Other Homologies: Human (97% sequence homology).
This antibody does not cross react with GABA(B)R1.

Physical form

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Analysis Note

Control
Rat microsomal brain tissue
Evaluated by Western Blotting in rat microsomal brain tissue.

Western Blotting: 1 µg/mL of this antibody detected GABA(B)R1 in 10 µg of rat microsomal brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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