MABN492
Anti-GABA(B)R1 Protein Antibody, clone N93A/49
clone N93A/49, from mouse
Synonym(s):
Gamma-aminobutyric acid type B receptor subunit 1, GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
N93A/49, monoclonal
species reactivity
mouse, rat
technique(s)
immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Gabbr1(54393)
rat ... Gabbr1(81657)
General description
Gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.
~115 kDa observed. Five isoforms at 112 kDa (Isoform 1E), 108 kDa (Isoform 1A), 95 kDa (Isoform 1B), 98 kDa (Isoform 1C) and 91 kDa (Isoform 1D) are known to exist. Uncharacterized bands may be observed above ~150 kDa in some tissue lysates.
Immunogen
Recombinant protein corresponding to rat GABA(B)R1.
Application
Immunohistochemistry Analysis: A representative lot detected GABA(B)R1 in adult rat hippocampus tissue (Prof. J. Trimmer, University of California, Davis).
Western Blotting Analysis: A representative lot detected GABA(B)R1 in adult rat brain tissue and in WT GABA(B)R1 mice, but demonstrated a loss of signal in GABA(B)R1 knockout mice.
Western Blotting Analysis: A representative lot detected GABA(B)R1 in adult rat brain tissue and in WT GABA(B)R1 mice, but demonstrated a loss of signal in GABA(B)R1 knockout mice.
This GABA(B)R1 Protein antibody is validated for use in WB & IHC for the detection of the GABA(B)R1 Protein protein.
Biochem/physiol Actions
Other Homologies: Human (97% sequence homology).
This antibody does not cross react with GABA(B)R1.
Physical form
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.
Analysis Note
Control
Rat microsomal brain tissue
Rat microsomal brain tissue
Evaluated by Western Blotting in rat microsomal brain tissue.
Western Blotting: 1 µg/mL of this antibody detected GABA(B)R1 in 10 µg of rat microsomal brain tissue.
Western Blotting: 1 µg/mL of this antibody detected GABA(B)R1 in 10 µg of rat microsomal brain tissue.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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