AB1783
Anti-Glutamate Transporter Antibody, Glial
serum, Chemicon®
Synonym(s):
GLT-1, EAAT2
About This Item
biological source
guinea pig
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC1A2(6506)
General description
EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
Immunogen
Application
Western Blot: 1:500 dilution of this antibody detected GLT-1 on 10 ug of mouse brain membrane lysates.
Immunohistochemistry:
1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.
Immunohistochemistry:
1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.
Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.
Notes:
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
Optimal working dilutions must be determined by the end user.
Neuroscience
Ion Channels & Transporters
Biochem/physiol Actions
Physical form
Preparation Note
Analysis Note
Rat brain tissue
Other Notes
Legal Information
Disclaimer
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Global Trade Item Number
SKU | GTIN |
---|---|
AB1783 | 04053252509391 |
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