Key Documents
AB5543
Anti-MAP2 Antibody
CHEMICON®, chicken polyclonal
Synonym(s):
Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Product Name
Anti-MAP2 Antibody, Chemicon®, from chicken
biological source
chicken
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
rat ... Map2(25595)
General description
Recognizes a doublet of ~280 kDa and a doublet of ~70kDa
Immunogen
Purified bovine brain MAP2
Application
Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.
Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.
Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.
Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive = cerebral cortex. Negative = liver or kidney.
Positive = cerebral cortex. Negative = liver or kidney.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5543 | 04053252403910 |
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