AB749P
Anti-Collagen Type I Antibody
Chemicon®, from rabbit
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
bovine
Application:
ELISA, IHC (p), RIA
Citations:
13
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, radioimmunoassay: suitable
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... COL1A1(1277)
Immunogen
purified bovine type I collagen extracted from purified bovine skin.
Application
Anti-Collagen Type I Antibody detects level of Collagen Type I & has been published & validated for use in ELISA, RIA, IH, IH(P).
Immunohistochemistry: 1:40 dilution for immunofluorescent staining of frozen bovine liver and skin tissues.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
Immunohistochemistry on 2% formalin or PFA fixed, paraffin embedded tissues requires HEIR antigen retrieval and enhanced enzymatic detection; methyl-carnoy, acetone, or acid-aldehyde fixation can enhance reactivity for paraffin sections.
Radioimmunoassay
ELISA: 1:200 direct
Not recommended for Western blots.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Reacts with bovine collagen type I collagen.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
Antibody shows less than 0.1% cross reactivity in RIA at 1:500 dilution with bovine collagen types II, IX & XI, or fibronectin; other collagens and ECM proteins untested.
Reactivity with human collagen type I <2.0% with RIA, 1:500; reactivity with Chicken collagen type I, less than 0.1% by RIA at 1:500 dilution.
Physical form
Format: Purified
Protein G purified IgG fraction in liquid at 1 mg/mL in 0.01M phosphate, 0.09M NaCl, pH 7.2.No preservatives.
Preparation Note
Maintain frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB749
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Telocytes/CD34+ Stromal Cells in the Normal, Hyperplastic, and Adenomatous Human Parathyroid Glands.
Lucio Díaz-Flores et al.
International journal of molecular sciences, 24(15) (2023-08-12)
Telocytes/CD34+ stromal cells (TCs/CD34+ SCs) have been studied in numerous organs and tissues, but their presence and characteristics in the parathyroid glands have not been explored. Using immunological and ultrastructural procedures, we assess the location, arrangement, and behavior of TCs/CD34+
Lucio Díaz-Flores et al.
Scientific reports, 10(1), 4987-4987 (2020-03-21)
In lobular capillary hemangioma (LCH), misnamed pyogenic granuloma, only sprouting angiogenesis (SA) has been considered. We assess the occurrence of intussusceptive angiogenesis (IA) in LCH and whether IA determines the specific and other focal patterns in the lesion. For this
Meng Wu et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 25(3), 822-829 (2019-04-27)
Tibetan mural samples from the Jiazhaer mountain cave were studied using enzyme-linked immunosorbent assays (ELISA) and immunofluorescence microscopy (IFM). Samples containing protein binders were first identified using ELISA, and then IFM was used to determine the location of protein binders.