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Merck

AB769

Anti-Collagen Type IV Antibody

Chemicon®, from goat

Synonym(s):

Anti-Anti-BSVD, Anti-Anti-BSVD1, Anti-Anti-COL4A1s, Anti-Anti-PADMAL, Anti-Anti-RATOR

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-Collagen Type IV Antibody, Chemicon®, from goat

biological source

goat

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, bovine, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

suitability

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_001845.4

UniProt accession no.

P02462

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... COL4A1(1282)

Analysis Note

Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia

Application

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody validated for use in DB, ELISA, IC, IH.
Dot and slot blotting: 1:100-1:500

ELISA: 1:1000-1:4000

Indirect immunohistochemistry (frozen sections only): 1:10-1:40

Immunocytochemistry 1:10-1:20

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Biochem/physiol Actions

Recognizes Human and bovine type IV collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types I, II, III, V, and VI. May show reactivity to type IV collagen from other species. AB769 has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human and bovine placental collagen type IV

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Prior to purification the antisera was adsorbed against collagen type I, II, III, V and VI immobilized on Sepharose™ 4B. Liquid in borate buffered saline, pH 8.2, no sodium azide.
ImmunoAffinity Purified

Preparation Note

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H360FD

Hazard Classifications

Repr. 1B

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Abigail L Mackey et al.
Skeletal muscle, 7(1), 24-24 (2017-11-09)
While muscle regeneration has been extensively studied in animal and cell culture models, in vivo myogenesis in adult human skeletal muscle has not been investigated in detail. Using forced lengthening contractions induced by electrical stimulation, we induced myofibre injury in
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Larsen, HS; Aure, MH; Peters, SB; Larsen, M; Messelt, EB; Kanli Galtung, H
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Driskell, I; Oda, H; Blanco, S; Nascimento, E; Humphreys, P; Frye, M
The Embo Journal null
Miguel Alejandro Lopez-Ramirez et al.
The Journal of experimental medicine, 214(11), 3331-3346 (2017-10-04)
KRIT1 mutations are the most common cause of cerebral cavernous malformation (CCM). Acute Krit1 gene inactivation in mouse brain microvascular endothelial cells (BMECs) changes expression of multiple genes involved in vascular development. These changes include suppression of Thbs1, which encodes
William P Daley et al.
Development (Cambridge, England), 139(2), 411-422 (2011-12-22)
The basement membrane is crucial for epithelial tissue organization and function. However, the mechanisms by which basement membrane is restricted to the basal periphery of epithelial tissues and the basement membrane-mediated signals that regulate coordinated tissue organization are not well
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Global Trade Item Number

SKUGTIN
AB76904053252508097

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