Anti-VAMP-7 Antibody

Vesicle-associated membrane protein 7 (VAMP-7), also known as Synaptobrevin-like protein 1 or Tetanus-insensitive VAMP (Ti-VAMP), and encoded by the gene name VAMP7 or SYBL1, is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. VAMP-7 is localized to late endosomes and lysosomes and is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP-7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP-7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.

~25 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human VAMP-7.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VAMP-7 in HeLa and PC12 cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected VAMP-7 in human kidney, human thalamus, and human cerebral cortex tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-VAMP-7 Antibody is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of VAMP-7.

This antibody detects VAMP-7a (isoform 1) and is expected to cross-react with VAMP-7b (isoform 2), but not VAMP-7c (isoform 3).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected VAMP-7 in 10 µg of rat brain tissue lysate.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.