ABD69
Anti-Nestin, Human Antibody
serum, from rabbit
Synonym(s):
Nestin
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
should not react with
rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... NES(10763)
General description
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.
The anti-Nestin antibody has an observed MW at 220kDa Antibody may see 260kDa band in some cells and/or treatments
Immunogen
GST-tagged recombinant protein corresponding to human Nestin.
Application
Detect Nestin, Human using this Anti-Nestin, Human Antibody validated for use in Western Blotting, ICC & IHC.
Evaluated by Immunocytochemistry in human WI38 cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human WI38 cells.
Evaluated by Immunohistochemistry in human glioblastoma tissue.
Immunocytochemsitry Analysis: A 1:2,000 dilution of this antibody detected Nestin in human glioblastoma tissue.
Evaluated by Western Blot in human U251 glioblastoma cells. Western Blot Analysis: A 1:25,000 dilution of this antibody has been shown to detected Nestin in human U251 glioblastoma cells.
Biochem/physiol Actions
This antibody recognizes human Nestin. Antibody is not reactive with rat and mouse Nestin protein.
Analysis Note
Control
U251 glioblastoma cells.
U251 glioblastoma cells.
Evaluated by Immunocytochemistry in human U251 glioblastoma cells. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected Nestin in human U251 glioblastoma cells.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Sutapa Ray et al.
Molecular carcinogenesis, 57(4), 536-548 (2017-12-28)
Medulloblastoma (MB) is a malignant pediatric brain tumor with poor prognosis. Signal transducers and activators of transcription-3 (STAT3) is constitutively activated in MB where it functions as an oncoprotein, mediating cancer progression and metastasis. Here, we have delineated the functional
Smita Jagtap et al.
Human molecular genetics, 28(21), 3625-3636 (2019-09-14)
The X-linked neurodevelopmental diseases CDKL5 deficiency disorder (CDD) and Rett syndrome (RTT) are associated with intellectual disability, infantile spasms and seizures. Although mitochondrial dysfunction has been suggested in RTT, less is understood about mitochondrial function in CDD. A comparison of
Intraspinal transplantation of mouse and human neural precursor cells.
Weinger, JG; Chen, L; Coleman, R; Leang, R; Plaisted, WC; Loring, JF; Lane, TE
Current Protocols in Stem Cell Biology null
Paul M George et al.
Biomaterials, 142, 31-40 (2017-07-19)
Exogenous human neural progenitor cells (hNPCs) are promising stroke therapeutics, but optimal delivery conditions and exact recovery mechanisms remain elusive. To further elucidate repair processes and improve stroke outcomes, we developed an electrically conductive, polymer scaffold for hNPC delivery. Electrical
Zong-Yu Xiao et al.
Turkish neurosurgery, 28(6), 910-914 (2018-01-26)
To investigate the expression of cancer stem cell markers in meningiomas. CD133, Nestin and Sox2 expression levels in 35 paraffin-embedded meningioma tissue samples were assessed using immunohistochemistry. In this study, five cases were atypical (WHO Grade II), two were anaplastic
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| ABD69 | 04053252694288 |
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