Skip to Content
Merck

ABE999

Anti-RPB3

from rabbit, purified by affinity chromatography

Synonym(s):

RPB3, DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, DNA-directed RNA polymerase II subunit C, RPB31

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

UniProt accession no.

P19387

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... POLR2C(5432)

General description

RPB3 is one of 12 subunits that make up the complete RNA polymerase, Pol II, the primary RNA polymerase in cellular metabolism. RPB3 is part of the core element of Pol II complex. RPB3 has a central large cleft and the clamp element that moves to open and close the cleft during RNA synthesis. RPB3 naturally localizes to the nucleus and expressed in all cell types and tissues. RPB3 also plays critical roles in RNA splicing and capping, key items of RNA function.
~35 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human RPB3 near the Near C-terminus.

Application

Anti-RPB3 Antibody is an antibody against RPB3 for use in Western Blotting.

Analysis Note

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected RPB3 in 10 µg of HUVEC cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Anne M Devlin et al.
Nature communications, 13(1), 7447-7447 (2022-12-03)
Control of RNA Polymerase II (pol II) elongation is a critical component of gene expression in mammalian cells. The PNUTS-PP1 complex controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol
Prashant Rawat et al.
Molecular cell, 81(5), 1013-1026 (2021-02-07)
In response to stress, human cells coordinately downregulate transcription and translation of housekeeping genes. To downregulate transcription, the negative elongation factor (NELF) is recruited to gene promoters impairing RNA polymerase II elongation. Here we report that NELF rapidly forms nuclear
Curtis W Bacon et al.
Molecular cell, 78(6), 1133-1151 (2020-05-14)
Precise control of the RNA polymerase II (RNA Pol II) cycle, including pausing and pause release, maintains transcriptional homeostasis and organismal functions. Despite previous work to understand individual transcription steps, we reveal a mechanism that integrates RNA Pol II cycle

Related Content

White Paper: Further considerations of antibody validation and usage.
New Products - Antibodies, Small Molecule, Inhibitors

"Cancer research giving you mixed signals? Signaling molecules are deeply embedded in processes associated with cancer. The study of signaling pathways is nearly synonymous with cancer research. Elucidating the various cellular and molecular mechanisms of cancer requires high quality research tools that give you precise, reproducible data. At EMD Millipore, our scientists are dedicated to developing and delivering thoroughly tested and validated tools for life science research."

NPI Flyer- Epigenetics and Nuclear Function Feature

Historically chromatin was considered to contain only DNA, histones, non-histone proteins and transiently associated mRNAs. While this remains true, current data suggest that a variety of non-coding RNAs (e.g. long non-coding RNAs, enhancer RNAs and even miRNAs) also associate with chromatin and serve regulatory functions.

Global Trade Item Number

SKUGTIN
ABE99904055977135350

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service