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Merck

ABS217

Anti-phospho-LAT Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Linker for activation of T-cells family member 1, 36 kDa phospho-tyrosine adapter protein, pp36, p36-38

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-phospho-LAT Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_055202

UniProt accession no.

O43561

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr200)

Quality Level

100

Gene Information

human ... LAT(27040)

Other Notes

Replaces: 04-467
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Analysis Note

Control
LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.
Evaluated by Western Blotting in LAT immunoprecipitated from untreated or CD3 treated Jurkat cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho LAT (Tyr200) immunoprecipitated from CD treated Jurkat cell lysates. A decrease in signal was observed against phospho LAT (Tyr200) immunoprecipitated from untreated Jurkat cell lysates.

Application

Detect LAT using this rabbit polyclonal antibody, Anti-phospho-LAT Antibody validated for use in western blotting.
Research Category
Signaling
Research Sub Category
Immunological Signaling
Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho LAT (Tyr200) in CD3 treated Jurkat cell lysates. However, a decrease in signal was observed against phospho LAT (Tyr200) in untreated Jurkat cell lysates.

Biochem/physiol Actions

Other homologies: Rat, Mouse, and Bovine (89% sequence homology).
This antibody recognizes the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of LAT phosphorylated at Tyrosine 200.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LAT (Linker for activation of T cells) is a transmembrane adaptor protein that plays an essential role in T cell receptor (TCR) signal transduction pathways. LAT is phosphorylated on multiple tyrosine residues upon TCR engagement, recruits several important signaling proteins to the membrane, and functions as a scaffold for the assembly of numerous signaling complexes.
~36 kDa observed. Uncharacterized bands may be observed in some cell lysates.

Immunogen

Epitope: Cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1
KLH-conjugated linear peptide corresponding to the cytoplasmic domain that interacts with GRB2, GRAP2 and PIK3R1 of human LAT phosphorylated at Tyrosine 200.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
ABS21704053252906336

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