AM63

Anti-A20 Mouse mAb (59A426)

Name: Anti-A20 Mouse mAb (59A426)
Brand: MM

liquid, clone 59A426, Calbiochem^®

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Product Name

Anti-A20 Mouse mAb (59A426), liquid, clone 59A426, Calbiochem^®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

59A426, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:500-1:2000)
Immunoprecipitation (2 µg/mg))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... TNFAIP3(7128)

Analysis Note

Positive Control
Daudi cells

Application

Immunoblotting (1:500-1:2000)
Immunoprecipitation (2 g/mg)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-A20 mouse monoclonal antibody, clone 59A426, recognizes ~70 kDa A20 protein in Daudi cells. It is validated for use in immunoblotting and immunoprecipitation.

Protein G purified mouse monoclonal antibody. Recognizes the ~70 kDa A20 protein.

Recognizes the ~70 kDa A20 protein in Daudi cells.

Immunogen

Human

full-length recombinant human A20 protein

Other Notes

Antibody should be titrated for optimal results in individual systems.

Heyninck, K., et al. J. Cell Biol145, 1471.
May, M.J. et al. 1997. Seminars in Cancer Biology.2, 63.
Tewari, M., et al. 1995. J. Immunol.154, 1699.
Opipari, A.W., et al. 1990. J. Biol. Chem.265, 14705.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.2% gelatin.

Preparation Note

Following initial thaw, aliquot freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Specific recognition of linear polyubiquitin by A20 zinc finger 7 is involved in NF-κB regulation.

Fuminori Tokunaga et al.

The EMBO journal, 31(19), 3856-3870 (2012-10-04)

LUBAC (linear ubiquitin chain assembly complex) activates the canonical NF-κB pathway through linear polyubiquitination of NEMO (NF-κB essential modulator, also known as IKKγ) and RIP1. However, the regulatory mechanism of LUBAC-mediated NF-κB activation remains elusive. Here, we show that A20

ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-κB.

Minho Won et al.

Cell death and differentiation, 29(6), 1152-1163 (2021-11-29)

In TNF signaling, ubiquitination of RIP1 functions as an early cell-death checkpoint, which prevents the spatial transition of the signaling complex from complex-I to death-inducing complex-II. Here, we report that ankyrin repeat domain 13a (ANKRD13a) acts as a novel component