AM80

Anti-PAR (Ab-1) Mouse mAb (10H)

Name: Anti-PAR (Ab-1) Mouse mAb (10H)
Brand: MM

liquid, ≥95% (SDS-PAGE), clone 10H

Synonym(s):

Anti-Poly (ADP-Ribose)

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Anti-PAR (Ab-1) Mouse mAb (10H), liquid, ≥95% (SDS-PAGE), clone 10H

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

10H, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, Drosophila, rat, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (2.5 µg/mL)
Immunocytochemistry (5-20 µg/mL))

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Negative Control
Normal mouse IgG

Positive Control
Etoposide-treated HL-60 cells

Application

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Disclaimer

Toxicity: Standard Handling (A)

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.

Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine

purified poly(ADP-ribose)

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.

PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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