CP70

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

Name: Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)
Brand: MM

liquid, clone FBN11, Calbiochem^®

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About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Product Name

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11), liquid, clone FBN11, Calbiochem^®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

FBN11, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

rat, human, mouse

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

dilution

(Frozen Sections (0.5-1 µg/mL)
Immunoblotting (0.5-1 µg/mL)
Immunofluorescence
Immunoprecipitation (2 µg/mg protein lysate)
Paraffin Sections (0.5-1 µg/mL, heat pre-treatment required))

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... FN1(2335)
mouse ... Fn1(14268)
rat ... Fn1(25661)

Analysis Note

Positive Control
SW 156 cells or normal kidney tissue

Application

Frozen Sections (0.5-1 µg/ml)

Immunoblotting (0.5-1 µg/ml)

Immunofluorescence (see comments)

Immunoprecipitation (2 µg/mg protein lysate)

Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).

Protein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.

Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.

Immunogen

recombinant human fibronectin

Other Notes

Clark, R.A. 2001. Ann. N.Y. Acad. Sci.936, 355.
Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta1470, O9.
Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum.29, 252.
Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
Rouslahti, E. and Pierschbacher, M.D. 1986. Cell44, 517.
Pytela, R., et al. 1985. Cell40, 191.

Exhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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