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Merck

EQCYTMAG-93K

MILLIPLEX® Equine Cytokine/Chemokine Panel

Configurable Equine Cyokine/Chemokine 23-Plex Panel

Synonym(s):

Equine cytokine panel, Luminex equine cytokine multiplex kit, Millipore horse cytokine kit, horse inflammation assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000
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species reactivity

horse

Quality Level

assay range

sensitivity: 16.7 pg/mL
(IP-10), sensitivity: 165 pg/mL
(IFNγ), sensitivity: 25.3 pg/mL
(IL-18), sensitivity: 29.3 pg/mL
(IL-1β), sensitivity: 3.9 pg/mL
(RANTES/CCL5), sensitivity: 3.94 pg/mL
(GM-CSF), sensitivity: 355 pg/mL
(IL-4), sensitivity: 39-130 pg/mL
(IL-13), sensitivity: 39.1 pg/mL
(IL-1α), sensitivity: 399 ng/mL
(MCP-1), sensitivity: 49.2 pg/mL
(IL-12 (p70)), sensitivity: 5.2 pg/mL
(TNFα), sensitivity: 62.5 pg/mL
(IL-10), sensitivity: 7.8 pg/mL
(GRO), sensitivity: 74.3 pg/mL
(IL-8/CXCL8), standard curve range: 15-15,000 pg/mL
(IL-17A), standard curve range: 15-15,000 pg/mL
(IL-2), standard curve range: 15-15,000 pg/mL
(IL-5), standard curve range: 15-15,000 pg/mL
(IL-6), standard curve range: 15-15,000 pg/mL
(IP-10), standard curve range: 29-30,000 pg/mL
(IL-1β), standard curve range: 293-300,000 pg/mL
(IL-4), standard curve range: 34-35,000 pg/mL
(IL-18), standard curve range: 342-350,000 ng/mL
(MCP-1), standard curve range: 39-40,000 pg/mL
(IL-12 (p70)), standard curve range: 39-40,000 pg/mL
(IL-13), standard curve range: 4-4,000 pg/mL
(TNFα), standard curve range: 488-500,000 pg/mL
(IFNγ), standard curve range: 49-50,000 pg/mL
(IL-10), standard curve range: 5-5,000 pg/mL
(GM-CSF), standard curve range: 5-5,000 pg/mL
(RANTES/CCL5), standard curve range: 59-60,000 pg/mL
(IL-1α), standard curve range: 59-60,000 pg/mL
(IL-8/CXCL8), standard curve range: 6-6,000 pg/mL
(GRO), accuracy: 74%
(IL-17a), accuracy: 76%
(IL-6), accuracy: 78%
(FGF-2), accuracy: 82%
(G-CSF), accuracy: 83%
(IFNγ), accuracy: 83%
(IL-12 (p70)), accuracy: 85%
(IL-13), accuracy: 86%
(IL-10), accuracy: 88%
(RANTES/CCL5), accuracy: 89%
(IL-8/CXCL8), accuracy: 90%
(IL-2), accuracy: 90%
(MCP-1), accuracy: 91%
(Fractalkine/CS3CL1), accuracy: 92%
(IL-5), accuracy: 93%
(Eotaxin/CCL11), accuracy: 97%
(GRO), sensitivity: 1.4 pg/mL
(IL-6), sensitivity: 10.6 pg/mL
(IL-5), sensitivity: 13.5 pg/mL
(IL-2), sensitivity: 15.4 pg/mL
(IL-17A)

technique(s)

multiplexing: suitable

input

serum plasma (Cell culture, cell culture supernatants)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

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General description

Researching horse cytokines and chemokines is pivotal for advancing our understanding of equine immunology and the underlying mechanisms of various diseases. By investigating the roles and interactions of these signaling molecules, scientists can uncover critical insights into inflammatory processes, immune responses, and the pathophysiology of equine conditions. This knowledge not only enhances our fundamental understanding of equine biology but also drives the development of targeted therapeutic strategies and interventions in equine medicine.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 23 analytes in equine serum, plasma, and cell culture supernatants.

Analytes included: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

  • Comprehensive 23-plex Cytokine and Chemokine Panel: Simultaneously quantify 23 critical immune mediators (including Eotaxin, FGF-2, G-CSF, IL-1α, IL-6, IL-10, TNFα, and more) in equine serum, plasma, or culture supernatant samples, providing a holistic view of the equine immune response.
  • High-Throughput 96-well Format: Analyze up to 38 samples in duplicate, maximizing laboratory efficiency and sample utilization while minimizing reagent costs and hands-on time.
  • In-Depth Immune Profiling: Investigate the immune response in equine models of infectious diseases, osteoarthritis, respiratory diseases, and other conditions, facilitating a better understanding of disease mechanisms and potential therapeutic targets.
  • Enhanced Statistical Power: Generate multi-parameter datasets from individual samples, improving experimental design and enabling sophisticated statistical analyses for publication-quality research outcomes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3


Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
EQCYTMAG-93K-0904055977358261
EQCYTMAG-93K-02C04054839177637
EQCYTMAG-93K-12C04054839177804
EQCYTMAG-93K-22C04054839177903
EQCYTMAG-93K-01C04054839177620
EQCYTMAG-93K-05C04054839177736
EQCYTMAG-93K-04C04054839177651
EQCYTMAG-93K-11C04054839177798
EQCYTMAG-93K-1204055977358407
EQCYTMAG-93K-0304055977358216
EQCYTMAG-93K-15C04054839177835
EQCYTMAG-93K-1504055977358377
EQCYTMAG-93K-21C04054839177897
EQCYTMAG-93K-2104055977358315
EQCYTMAG-93K-20C04054839177880
EQCYTMAG-93K-2304055977358292
EQCYTMAG-93K-18C04054839177866
EQCYTMAG-93K-0804055977358254
EQCYTMAG-93K-1904055977358339
EQCYTMAG-93K-0104055977357875
EQCYTMAG-93K-0204055977357882
EQCYTMAG-93K-0604055977358247
EQCYTMAG-93K-1004055977358278
EQCYTMAG-93K-06C04054839177743
EQCYTMAG-93K-0504055977358230

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