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EQCYTMAG-93K

Millipore

MILLIPLEX® Equine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Quality Level

200

species reactivity

horse

manufacturer/tradename

Milliplex®

assay range

accuracy: 74%
(IL-17a)
accuracy: 76%
(IL-6)
accuracy: 78%
(FGF-2)
accuracy: 82%
(G-CSF)
accuracy: 83%
(IFNγ)
accuracy: 83%
(IL-12 (p70))
accuracy: 85%
(IL-13)
accuracy: 86%
(IL-10)
accuracy: 88%
(RANTES/CCL5)
accuracy: 89%
(IL-8/CXCL8)
accuracy: 90%
(IL-2)
accuracy: 90%
(MCP-1)
accuracy: 91%
(Fractalkine/CS3CL1)
accuracy: 92%
(IL-5)
accuracy: 93%
(Eotaxin/CCL11)
accuracy: 97%
(GRO)
sensitivity: 1.4 pg/mL
(IL-6)
sensitivity: 10.6 pg/mL
(IL-5)
sensitivity: 13.5 pg/mL
(IL-2)
sensitivity: 15.4 pg/mL
(IL-17A)
sensitivity: 16.3 pg/mL
(FGF-2)
sensitivity: 16.7 pg/mL
(IP-10)
sensitivity: 165 pg/mL
(IFNγ)
sensitivity: 25.3 pg/mL
(IL-18)
sensitivity: 29.3 pg/mL
(IL-1β)
sensitivity: 3.9 pg/mL
(RANTES/CCL5)
sensitivity: 3.94 pg/mL
(GM-CSF)
sensitivity: 340 pg/mL
(G-CSF)
sensitivity: 355 pg/mL
(IL-4)
sensitivity: 39-130 pg/mL
(IL-13)
sensitivity: 39.1 pg/mL
(IL-1α)
sensitivity: 399 ng/mL
(MCP-1)
sensitivity: 457 pg/mL
(Fractalkine/CS3CL1)
sensitivity: 49.2 pg/mL
(IL-12 (p70))
sensitivity: 5.2 pg/mL
(TNFα)
sensitivity: 62.5 pg/mL
(IL-10)
sensitivity: 7.8 pg/mL
(GRO)
sensitivity: 74.3 pg/mL
(IL-8/CXCL8)
sensitivity: 8.3 pg/mL
(Eotaxin/CCL11)
standard curve range: 15-15,000 pg/mL
(IL-17A)
standard curve range: 15-15,000 pg/mL
(IL-2)
standard curve range: 15-15,000 pg/mL
(IL-5)
standard curve range: 15-15,000 pg/mL
(IL-6)
standard curve range: 15-15,000 pg/mL
(IP-10)
standard curve range: 16.3 pg/mL
(FGF-2)
standard curve range: 29-30,000 pg/mL
(IL-1β)
standard curve range: 293-300,000 pg/mL
(IL-4)
standard curve range: 34-35,000 pg/mL
(IL-18)
standard curve range: 340 pg/mL
(G-CSF)
standard curve range: 342-350,000 ng/mL
(MCP-1)
standard curve range: 39-40,000 pg/mL
(IL-12 (p70))
standard curve range: 39-40,000 pg/mL
(IL-13)
standard curve range: 4-4,000 pg/mL
(TNFα)
standard curve range: 457 pg/mL
(Fractalkine/CS3CL1)
standard curve range: 488-500,000 pg/mL
(IFNγ)
standard curve range: 49-50,000 pg/mL
(IL-10)
standard curve range: 5-5,000 pg/mL
(GM-CSF)
standard curve range: 5-5,000 pg/mL
(RANTES/CCL5)
standard curve range: 59-60,000 pg/mL
(IL-1α)
standard curve range: 59-60,000 pg/mL
(IL-8/CXCL8)
standard curve range: 6-6,000 pg/mL
(GRO)
standard curve range: 8.3 pg/mL
(Eotaxin/CCL11)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

Related Categories

General description

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for horses as it is for humans. Both experience immunological diseases, such as infectious diseases, osteoarthritis, and respiratory diseases, as well as neurologic, metabolic, and cardiovascular diseases and cancer.

MILLIPLEX® Equine Cytokine/Chemokine Premix Panel is a 23-plex kit used for the simultaneous quantification of any or all the following analytes in equine serum, plasma, or culture supernatant samples: Eotaxin/CCL11, FGF-2, Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Application

  • Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α
  • Recommended Sample Type: Equine serum, plasma, tissue culture supernatant or tissue/cell extracts
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require dilution with an appropriate medium
  • Assay Run Time: A primary incubation of 2 hours at room temperature (RT) (20-25°C), or for greater sensitivity, overnight (16-18 hours) at 2-8°C; followed by a 1 hour secondary incubation at RT
  • Research Category: Inflammation & Immunology

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKUGTIN
EQCYTMAG-93K-01C04054839177620
EQCYTMAG-93K-2104055977358315
EQCYTMAG-93K-11C04054839177798
EQCYTMAG-93K-21C04054839177897
EQCYTMAG-93K-02C04054839177637
EQCYTMAG-93K-12C04054839177804
EQCYTMAG-93K-1204055977358407
EQCYTMAG-93K-22C04054839177903
EQCYTMAG-93K-0304055977358216
EQCYTMAG-93K-2304055977358292
EQCYTMAG-93K-20C04054839177880
EQCYTMAG-93K-18C04054839177866
EQCYTMAG-93K-0804055977358254
EQCYTMAG-93K-0904055977358261
EQCYTMAG-93K-05C04054839177736
EQCYTMAG-93K-15C04054839177835
EQCYTMAG-93K-04C04054839177651
EQCYTMAG-93K-1504055977358377
EQCYTMAG-93K-0104055977357875
EQCYTMAG-93K-1104055977358285
EQCYTMAG-93K-0204055977357882
EQCYTMAG-93K-2204055977358308
EQCYTMAG-93K-1304055977358391
EQCYTMAG-93K-03C04054839177644
EQCYTMAG-93K-13C04054839177811

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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