HTS210RTA
Ready-to-Assay P2Y2 Purinergic Receptor Frozen Cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
eCl@ss:
32011203
Quality Segment
description
Cell line type: GPCR Cell Lines, GPCR Class A, Host Cells 1321N1, protein target P2Y2
technique(s)
calcium flux assay: suitable
detection method
fluorometric
General description
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
The P2Y GPCRs serve as receptors for extracellular nucleotides (Abbracchio et al., 2006). P2Y2 (also known as P2U) is activated by UTP and ATP, and couples to Gq to increase intracellular calcium (Parr et al., 1994). The P2Y2 receptor knockout mouse displays defective nucleotide-stimulated Cl- secretion, and a P2Y2 selective agonist increases tracheal epithelial chloride and water secretion (Cressman et al., 1999; Yerxa et al., 2002). As a result, P2Y2 is a potential target for treatment of cystic fibrosis. In addition, the P2Y2 KO mouse displays salt-resistant hypertension (Rieg et al., 2007). Millipore′s cloned human P2Y2-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y2 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y2.
The P2Y GPCRs serve as receptors for extracellular nucleotides (Abbracchio et al., 2006). P2Y2 (also known as P2U) is activated by UTP and ATP, and couples to Gq to increase intracellular calcium (Parr et al., 1994). The P2Y2 receptor knockout mouse displays defective nucleotide-stimulated Cl- secretion, and a P2Y2 selective agonist increases tracheal epithelial chloride and water secretion (Cressman et al., 1999; Yerxa et al., 2002). As a result, P2Y2 is a potential target for treatment of cystic fibrosis. In addition, the P2Y2 KO mouse displays salt-resistant hypertension (Rieg et al., 2007). Millipore′s cloned human P2Y2-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y2 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y2.
Other Notes
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Disclaimer
This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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