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Merck

MAB1273A4

Anti-Mitochondria Antibody, clone 113-1, Alexa Fluor™ 488 conjugate

clone 113-1, from mouse, ALEXA FLUOR™ 488

Synonym(s):

Human Mitochondria

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 488
Clone:
113-1, monoclonal
Application:
ICC
Citations:
8
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biological source

mouse

Quality Segment

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

113-1, monoclonal

species reactivity

human (mitochondria protein)

should not react with

mouse (mitochondria protein), rat (mitochondria protein)

technique(s)

immunocytochemistry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

General description

60 kDa calculated
This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273A4 recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells.

Application

Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1 & Alexa Fluor™ 488 conjugate validated for use in ICC.
Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody did not detect mitrochondria in primary mouse embryonic fibroblasts (PMEFs).

Analysis Note

Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038).
Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies


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Storage Class

12 - Non Combustible Liquids

WGK

WGK 2



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