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MAB2605

Anti-ATG5 Antibody, clone 177.19

Name: Anti-ATG5 Antibody, clone 177.19
Brand: MM

clone 177.19, from mouse

Synonym(s):

APG5 autophagy 5-like, APG5 autophagy 5-like (S. cerevisiae), APG5 (autophagy 5, S. cerevisiae)-like, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5 autophagy related 5-like, Apoptosis-specific protein

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

177.19, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

NM_004849.2

UniProt accession no.

Q9H1Y0

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)

General description

50 kDa

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity

Immunogen

Linear Peptide, aa: 8-318

Application

Immunoprecipitation: HeLa cell lysate

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Biochem/physiol Actions

Other species have not been tested.

The antibody reacts with the 50 kDa ATG5 protein.

Physical form

Format: Purified

Protein G Purified

Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot and Immunoprecipitation: HeLa cell lysate.

Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABC137

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells.

Wen-Tsung Huang et al.

Frontiers in pharmacology, 8, 902-902 (2018-01-13)

Intrinsic or acquired resistance to hormone therapy is frequently reported in estrogen receptor positive (ER+) breast cancer patients. Even though dysregulations of histone deacetylases (HDACs) are known to promote cancer cells survival, the role of different HDACs in the induction

IRGM is a common target of RNA viruses that subvert the autophagy network.

Isabel Pombo Grégoire et al.

PLoS pathogens, 7(12), e1002422-e1002422 (2011-12-17)

Autophagy is a conserved degradative pathway used as a host defense mechanism against intracellular pathogens. However, several viruses can evade or subvert autophagy to insure their own replication. Nevertheless, the molecular details of viral interaction with autophagy remain largely unknown.

BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

Tzu-Yu Lin et al.

Autophagy, 16(7), 1296-1313 (2019-10-16)

BIRC5/Survivin is known as a dual cellular functions protein that directly regulates both apoptosis and mitosis in embryonic cells during embryogenesis and in cancer cells during tumorigenesis and tumor metastasis. However, BIRC5 has seldom been demonstrated as a direct macroautophagy/autophagy

Global Trade Item Number

SKU	GTIN
MAB2605	04053252398391

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