Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10

Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others. This antibody recognizes an epitope in amino acids 256-308 of LEF-1. This region is between the transactivation domain and the HMG binding domain of LEF-1 and is conserved in all LEF-1 isoforms.

Epitope: all isoforms

Recombinant full length LEF-1 protein.

Anti-LEF-1 Antibody, all isoforms, clone 1C3.1D10 is an antibody against LEF-1 for use in WB.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells

Western blot

Optimal working dilutions must be determined by end user.

Recognizes human LEF-1 (Lymphoid Enhancer Factor-1) protein. Does not cross-react with TCF-1 or TCF-4.

Format: Purified

Purified immunoglobulin. Provided as solution in phosphate buffered saline with 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Control
Jurkat cell lysate and human colon adenocarcinoma tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1130

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