MAB3864
Anti-DNA/Histone H1 Antibody
Chemicon®, from mouse
Synonym(s):
Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
monoclonal
Application:
ELISA, FACS, ICC, IHC
Citations:
39
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H1-1(3024)
Immunogen
Human Raji cell nuclei
Application
Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.
Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)
Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.
Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.
ELISA, direct and indirect
Optimal working dilutions must be determined by end user.
STAINING PATTERN:
Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.
Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.
ELISA, direct and indirect
Optimal working dilutions must be determined by end user.
STAINING PATTERN:
Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.
Physical form
Format: Purified
Protein A purified culture supernatant. Liquid in PBS
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Nicole de Buhr et al.
Cellular microbiology, 19(2) (2016-07-28)
Streptococcus suis is an important meningitis-causing pathogen in pigs and humans. Neutrophil extracellular traps (NETs) have been identified as host defense mechanism against different pathogens. Here, NETs were detected in the cerebrospinal fluid (CSF) of S. suis-infected piglets despite the presence
Neila Álvarez de Haro et al.
Fish & shellfish immunology, 119, 209-219 (2021-08-27)
Neutrophils release chromatin extracellular traps (ETs) as part of the fish innate immune response to counter the threats posed by microbial pathogens. However, relatively little attention has been paid to this phenomenon in many commercially farmed species, despite the importance
Nicole de Buhr et al.
Microbiology (Reading, England), 161(Pt 4), 838-850 (2015-02-11)
The porcine and human pathogen Streptococcus suis induces and degrades neutrophil extracellular traps (NETs) in vitro. In this study, we investigated the working hypothesis that NET degradation is mediated not only by the known secreted S. suis nuclease A (SsnA)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB3864 | 04053252612503 |