Anti-Casper/c-FLIP Antibody, clone 17B12.1

Caspase-eight-related protein (Casper), also known as Cellular FLICE-like inhibitory protein (c-FLIP), CASP8, FADD-like apoptosis regulator (CFLAR), Caspase homolog (CASH), Caspase-like apoptosis regulatory protein (CLARP), FADD-like antiapoptotic molecule 1 (FLAME-1), Inhibitor of FLICE (I-FLICE), MACH-related inducer of toxicity (MRIT), and Usurpin. Casper shows a wide expression profile, with higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Casper is an apoptosis regulator and acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Casper lacks enzymatic activity.

~55 kDa observed. Uniprot describes many isoforms produced by alternative splicing ranging from ~23 kDa through ~55 kDa

GST-tagged recombinant protein corresponding to human Casper/c-FLIP.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Casper/c-FLIP in human cardiac myocytes tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Casper/c-FLIP antibody is validated for use in WB & IHC for the detection of the Casper/c-FLIP protein.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
PC-12 cell lysate

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Casper/c-FLIP in 10 µg of PC-12 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.