MABC161
Anti-BRCA1 Antibody, clone MS13
clone MS13, from mouse
Synonym(s):
Breast cancer type 1 susceptibility protein, RING finger protein 53
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
MS13, monoclonal
Application:
ICC, IHC, WB
Citations:
2
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MS13, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... BRCA1(672)
General description
Breast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.
~220 kDa observed. The calculated molecular weight of this protein is 208 kDa, but can be observed at ~220 kDa due to high glycosylation. An uncharacterized band appears at ~65 kDa in some lysates.
Immunogen
Recombinant protein corresponding to human BRCA1.
Application
Anti-BRCA1 Antibody, clone MS13 detects level of BRCA1 & has been published & validated for use in Western Blotting, IHC, ICC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BRCA1 in human breast cancer and human ovarian cancer tissues.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected BRCA1 in HeLa and A431 cells.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected BRCA1 in HeLa and A431 cells.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected BRCA1 on 10 µg of HeLa nuclear extract.
Western Blot Analysis: 0.5 µg/mL of this antibody detected BRCA1 on 10 µg of HeLa nuclear extract.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins
Stephanie Panier et al.
Molecular cell, 47(3), 383-395 (2012-06-30)
The response to DNA double-strand breaks (DSBs) entails the hierarchical recruitment of proteins orchestrated by ATM-dependent phosphorylation and RNF8-mediated chromatin ubiquitylation. As in most ubiquitin-dependent processes, the ordered accumulation of DNA repair factors at the break site relies on ubiquitin-binding
S Fan et al.
Oncogene, 20(57), 8215-8235 (2002-01-10)
Unregulated expression of wild-type BRCA1 (wtBRCA1) confers an altered phenotype in cultured human prostate cancer cells, characterized by chemosensitivity, susceptibility to apoptosis, decreased DNA repair activity, and alterations of key cell regulatory proteins. We now report that the expression of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABC161 | 04053252595950 |