Anti-BIRC2/IAP-2, clone 1E1-1-10 Antibody

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected BIRC2/IAP-2 in 10 µg of NIH/3T3 cell lysate.

Anti-BIRC2/IAP-2 antibody, clone 1E1-1-10 is an antibody against BIRC2/IAP-2 for use in western blotting.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2 µg/mL from a representative lot detected BIRC2/IAP-2 in C2C12 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in HeLa and Raji cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in MEF cell lysate (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.).

Western Blotting Analysis: A representative lot from an independent laboratory detected BIRC2/IAP-2 in stable cell lines expressing EYFP cIAP1 RING, or EYFP cIAP1 RING (Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

BRC2/IAP-2, also known as IAP homolog B, Inhibitor of apoptosis protein 2 and Baculoviral IAP repeat-containing protein 2, and encoded by the gene BIRC2/API1/IAP2/MIHB/ RNF48, is a multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. BIRC2/IAP-2 also acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical pathways. Additionally, BIRC2/IAP-2 acts as an important regulator of innate immune signaling, and finally, BIRC2/IAP-2 plays an important role in the modulation of the cell cycle. BIRC2/IAP-21 is localized to nucleus except during apoptotic events. BIRC2/IAP-2 is expressed in fetal and adult tissues with a strong emphasis found in adult skeletal muscle, thymus, testis, ovary and pancreas with low or absent expression in blood and brain.

~65 kDa observed. The calculated molecular weight of this protein is 70 kDa; however, this protein has been observed at lower molecular weights (Gerlach, B., et al. (2011). Nature. 471(7340):591-596.; Silke, J., et al. (2005). Proc Natl Acad Sci USA. 102(45):16182-16187.).

Epitope: This antibody recognizes the BIR 2 repeat region of BIRC2/IAP-2.

Linear peptide corresponding to the BIR 2 repeat region of mouse BIRC2/IAP-2.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Stable for 1 year at 2-8°C from date of receipt.