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MABC541

Anti-Cullin-3 Antibody, clone 3A8.1

Name: Anti-Cullin-3 Antibody, clone 3A8.1
Brand: MM

clone 3A8.1, from mouse

Synonym(s):

Transient receptor potential cation channel subfamily V member 4, TrpV4, Osm-9-like TRP channel 4, OTRPC4, Transient receptor potential protein 12, TRP12, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, VRL-2, Vanilloid receptor-rel

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

3A8.1, monoclonal

Application:

Citations:

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Properties

biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3A8.1, monoclonal

species reactivity

monkey, mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_003581

UniProt accession no.

Q13618

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CUL3(8452)

Description

General description

Cullin-3 (UniProt Q13618) is encoded by the CUL3 (also known as PHA2E, KIAA0617) gene (Gene ID 8452) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Cullin-3 belongs to the cullin family of hydrophobic proteins that confer substrate specificity to the multimeric Cullin-RING E3 ubiquitin ligase (CRL) complexes by acting as scaffold proteins. The activity of CRLs is regulated by Cullin Neddylation via the reversible conjugation of a small ubiquitin-type protein known as Neural precursor cell expressed developmentally down-regulated protein 8 (Nedd8). Cullin-3-based CRLs rely on Cullin-3 interaction with bric-a-brac/tramtrack/broad-complex (BTB) domain-containing proteins for targets recruitment.

~88 kDa observed. Uncharacterized band(s) may appear in some lysates.

Immunogen

Epitope: C-terminal

GST-tagged recombinant protein corresponding to the C-terminal of human Cullin-3.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Cullin-3 Antibody, clone 3A8.1 is validated for use in Western Blotting for the detection of Cullin-3.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cullin-3 in 10 µg of mouse neuroblastoma Neuro-2a (N2a) cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c